Coding

Part:BBa_K3286283

Designed by: Santiago Castanedo Fontanillas   Group: iGEM19_Wageningen_UR   (2019-10-21)


Lambda gpD - ribosomal frameshifting sequence - Chitinase A1

This part can be used for the constitutive expression of a fusion gene that cosists of : - 6X-His tag at the N-terminus of the protein, used to quantify the amount of expressed fusion protein via Western blot. - gpd gene (encodes the lambda decorator protein) from the lambda phage. - A ribosomal frameshifting sequence that links the two proteins together. - Chitinase A gene so that eventually, if engineered with this fusion protein, phages can display the fusion protein on the capsid and therefore, phages can bind to chitin. The promoter is very strong (J23100), as well as the ribosome binding site (RBS).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None